Table 3:

Reagents details

Antibodies used for immunocytochemistry/flow-cytometry
	Antibody	Dilution	Company Cat # and RRID
Pluripotency Markers	Mouse anti-SOX2	1:200	Millipore, Cat# MAB4423, RRID:AB_11213224
Pluripotency Markers	PE anti-human SSEA-4	1:200	BioLegend Cat# 330406, RRID:AB_1089206
Pluripotency Markers	Mouse anti-OCT3/4	1:200	Santa Cruz Biotechnology Cat# sc5279, RRID: AB_628051
Pluripotency Markers	Rabbit anti-OCT4	1:200	Abcam Cat #ab19557, RRID:N/A
Pluripotency Markers	Mouse anti-Nanog	1:200	Millipore Cat# MABD24, RRID:AB_11203826
Differentiation Markers	Goat anti-SOX17	1:200	R and D Systems Cat# AF1924, RRID:AB_355060
Differentiation Markers	Goat anti-Brachyury	1:200	R and D Systems Cat# AF2085, RRID:AB_2200235
Differentiation Markers	Chicken anti-beta-III Tubulin	1:200	Novus Cat# NB100–1672, RRID:AB_522025
Secondary antibodies	Donkey anti-mouse Alexa Fluor 488	1:500	Thermo Fisher Scientific Cat# A21202, RRID:AB_141607
Secondary antibodies	Donkey anti-mouse Alexa Fluor 568	1:500	Thermo Fisher Scientific Cat# A10037, RRID:AB_2534013
Secondary antibodies	Donkey anti-rabbit Alexa Fluor 568	1:500	Thermo Fisher Scientific Cat# A10042, RRID:AB_2534017
Secondary antibodies	Donkey anti-goat Alexa Fluor 488	1:500	Thermo Fisher Scientific Cat# A11055, RRID:AB_2534102
Secondary antibodies	Goat anti-chicken Alexa Fluor 568	1:500	Thermo Fisher Scientific Cat# A11041, RRID:AB_2534098
Primers
	Target	Forward/Reverse primer (5′−3′)
Targeted variant analysis/sequencing	A53T	CTAGCTAATCAGCAATTTAAGGCTA/GCTC AGTGATTGTTTTACAATTTCA
CRISPR reagents
Targeted mutation	crRNA used for Cas9 editing	Donor oligo sequence
A53T	GTGGTGCATGGTGTGACAAC agg	AAAACTAGCTAATCAGCAATTTAAGGCTAGCTT GAGACTTATGTCTTGAATTTGTTTTTGTAGGCT CCAAAACCAAGGAGGGAGTGGTGCATGGTGT GGCAACAGGTAAGCTCCATTGTGCTTATATCC
		AAAGATGATATTTAAAGTATCTAGTGATTAGTG TGGCCCAGTATTCAAGATTCCTATGAAATTGTAAAAC