FIG. 3.

Lectin blot of virion polypeptides. IPNV was purified by an additional step of isodensity centrifugation after detergent treatment. Upon SDS-PAGE of the recovered virus and transblotting of the polypeptides to a nitrocellulose membrane, carbohydrates were monitored with 14 different lectins. The total amount of virion proteins subjected to SDS-PAGE was 67 ng per channel (42). The specificities of the different lectins are explained in Table 1. The positions of the viral proteins were detected with a polyclonal serum against IPNV (αIPNV). For definitions of abbreviations, see the text and Table 1, footnote a.