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. 2023 Jul 28;19(7):e1011112. doi: 10.1371/journal.ppat.1011112

Fig 4. EPCT is indispensable during the promastigote stage.

Fig 4

(A) Promastigotes were continuously cultivated in the presence or absence of ganciclovir (GCV) or nourseothricin (SAT) and passed every three days. Percentages of GFP-high cells were determined for every passage. Error bars represent standard deviations from three repeats (**: p < 0.01, ***: p < 0.001). (B-D) After 14 passages, WT (B) and epct¯+pXNG4-EPCT parasites grown in the presence of SAT (C) or GCV (D) were analyzed by flow cytometry to determine the percentages of GFP-high cells (indicated by R2 in the histograms). (E-F) Two clones were isolated from the GFP-low population in D by FACS, amplified in the absence of SAT and presence of GCV, and analyzed for GFP expression levels by flow cytometry (more details for the sorting were included in S5 Fig).