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. 2023 Jul 28;19(7):e1011112. doi: 10.1371/journal.ppat.1011112

Fig 6. Expression level of EPCT mRNA in promastigotes and amastigotes.

Fig 6

Total RNA was extracted from promastigotes (Pro) or amastigotes and the relative EPCT transcript levels were determined by qRT-PCR using the ΔΔCt method with the α-tubulin gene as the internal control. (A) WT promastigotes and WT amastigotes (6 weeks post infection). (B) Promastigotes grown in the absence (WT and EPCT+/-) or presence of SAT (WT+pXNG4-EPCT, EPCT+/- +pXNG4-EPCT and epct¯+pXNG4-EPCT). (C) Lesion derived amastigotes from mice treated with GCV or PBS (weeks 6 for WT and EPCT+/-, week 14–20 for EPCT overexpressors). Error bars represent standard deviation from three independent repeats (**: p < 0.01, ***: p < 0.001).