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. 2023 Aug 9;9(32):eadh0066. doi: 10.1126/sciadv.adh0066

Table 1. List of the aerobic traits considered for the analysis in Fig. 2.

AOX, alternative oxidase.

Protein and defining trait Premium for synteny* Penalty for absence Considered earlier
Cyt C cytochrome c No, this work
COX1 COX – complex IV +2 with COX2 −1 Yes
COX2 COX – complex IV −1 Yes
COX3 COX – complex IV +2 with COX11 Yes
Cox11 COX assembly Yes
Cox15 type2, heme A synthesis Yes
SURF1 COX assembly Yes
SCO COX assembly Numeral as its multiple genes Yes
Zf-CHCC precursor subunit Vb No, this work
M16B precursor of MPP +2 with ISP 0 if not close to COX No, this work
Cbp3 bc1 assembly No, this work
ISP bc1 – complex III Yes
CytB bc1 – complex III Yes
CytC1 bc1 – complex III Yes
CcmF cyt C biosynthesis +1 with two other Ccm genes Yes
CcmE cyt C biosynthesis No, this work
CcmA cyt C biosynthesis No, this work
AOX alternative oxidase Numeral as its multiple genes Yes
M16A fused zinc peptidases Capped numeral for multiple genes No, this work
Cys near C terminus COX1 No, this work

*Numerical premium given to collinear synteny, as described in the Materials and Methods.

†A few alphaproteobacterial genomes have multiple genes for SCO proteins, while some eukaryotes have multiple AOX genes.

‡Several eukaryotes have up to eight M16 (A, B, and C subfamily) peptidases, but their maximal numeral was capped at five as described in Materials and Methods.