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. 2022 Aug 20;119(5):1218–1233. doi: 10.1093/cvr/cvac137

Figure 1.

Figure 1

Effects of aspirin, COX-2 inhibitor, and mPGES-1 on recovery from hindlimb ischaemia. (A–G) From the day of surgery, C57Bl/6 mice received oral administration of aspirin, a selective COX-2 inhibitor, or PBS daily. (A) Aspirin impaired blood flow recovery from ischaemia. Data are expressed as means ± standard deviation (SD) of the n = 4–7 mice/group. *P < 0.05 vs. vehicle, repeated-measures ANOVA (upper panel). Representative Doppler images 28 days after ligation (lower panel). (B) CD31 staining of ischaemic limbs 28 days after ligation. Triangles indicate CD31+ cells. Scale bar, 50 μm. (C) Number of CD31+ cells in ischaemic muscle tissue 28 days after ligation. Data are expressed as means ± SD of the n = 4–7 mice/group. *P < 0.05 vs. PBS, Student’s t-test. (D) The selective COX-2 inhibitor, celecoxib, impaired blood flow recovery from ischaemia. Data are means ± SD of the n = 5–6 mice/group. *P < 0.05 vs. vehicle, repeated-measures ANOVA (upper panel). Representative Doppler images 28 days after ligation (lower panel). (E) CD31 staining of the ischaemic limb 28 days after ligation. Triangles indicate CD31+ cells. Scale bar, 50 μm. (F) Number of CD31+ cells in ischaemic muscle tissue 28 days after ligation. Data are means ± SD of the n = 5–6 mice/group. *P < 0.05 vs. PBS, Student’s t-test. (G) mPges-1 mRNA expression in ischaemic muscle tissue. Aspirin- and celecoxib-treated mice exhibited decreased mRNA expression of mPges-1 compared with PBS. Data are expressed means ± SD of the n = 4 mice/group. *P < 0.05, one-way ANOVA. (H) Ischaemia was induced in the left hindlimb of C57Bl/6 mice (wild-type, WT) and mPges-1−/− mice. mPges-1−/− mice ablation impaired blood flow recovery from ischaemia. Data are means ± SD of the n = 5 mice/group. *P < 0.05 vs. WT, repeated-measures ANOVA (upper panel). Representative Doppler images 28 days after ligation (lower panel). (I) Number of CD31+ cells in ischaemic limbs 28 days after ligation. Triangles indicate CD31+ cells. Scale bar, 50 μm. (J) Number of CD31+ cells in ischaemic muscle tissue 28 days after ligation. Data are means ± SD of the n = 7 mice/group. *P < 0.05 vs. PBS, Student’s t-test.