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. 2023 Jul 27;12:e84679. doi: 10.7554/eLife.84679

Table 1. Biological process (BP) of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for differentially enriched genes in each inflammatory cell clusters.

Cluster GO-BP term Enriched genes KEGG Enriched genes
Mac 1 Vesicle-mediated transport cation transport havcr1, marco, igf2r Endocytosis igf2r
Proton transmembrane transporter Ferroptosis Heme metabolism (hmox1a)
(atp6v0ca, atp6v1e1b)
Solute carrier family (slc2a6, slc30a1a)
Mac 2 Oxygen transport Hemoglobins (hb genes), myoglobin (mb) Unclassified Heme metabolism (hmox1a)
Hydrogen peroxide metabolic process Hemoglobins (hb genes)
Mac 3 Immune system process cd74a, csf1ra Ferroptosis Heme metabolism (hmox1a)
regulation of cell differentiation lgals2a, spi1a Apoptosis Pro-apoptotic gene (pmaip1)
Unclassified timp4.3
Mac 4 Response to oxidative stress prdx2, anxa1a Citrate cycle (TCA cycle) TCA cycle-related genes
(suclg1, suclg2, mdh1aa, sdha, dlst)
Response to wounding cxcl8a, lgals2a
Mac 5 Mitotic cell cycle cdk1, top2a DNA replication pcna
Mac 6, 7 Response to oxidative stress anxa1a, prdx2, park7, hmox1a Cardiac muscle contraction Cytochrome c oxidase involved
in oxidative phosphorylation
Immune system development Neutrophil transmigration (anxa1a, cx43) Ribosome
Leukocyte differentiation (ak2)
Mac 8 Mitochondrion organization NADH dehydrogenase (ndufs1, ndufs8a) Cardiac muscle contraction tnnt2a, myh7l
Ubiquinol-cytochrome c reductase
(uqcr10, uqcrc2b)
Mitochondrial transport vdac2, uqcrc2a, cyc1 Glycolysis/gluconeogenesis aldoab, pdha1a
Muscle structure development pgam2, desma, csrp3 Fatty acid degradation acadvl, acsl1b
Heart development fabp3, myl7, nppa
Mac 9 Regulation of cell differentiation irf8, jun, myd88 Endocytosis cxcr4a, cxcr4b, spg21, eps15
Cellular macromolecule localization cd74a, cd74b Toll-like receptor signaling pathway Toll-like receptors (tlr3, tlr8b, tlr9)
Interferon-induced genes (irf5, irf7)
MN Immune response Inflammation-related genes (tnfb, irak3) Mitophagy Autophagy-involved genes (gabarapa, gabarapb, gabarapl2, calcoco2)
Response to cytokine Cytokines and receptors NOD-like receptor signaling pathway Interferon-induced genes (stat1b, irf1b)
(cxcl11.1, cxcl20, tnfrsf18, ccr9a)
C-type lectin receptor signaling pathway Fc receptor (fcer1g),
adaptor protein of PRR (card9)
Ferroptosis hmox1a
Neu 1 Unclassified sat1a.2, raraa Ribosome rplp0, rpsa, rpl15
Neu 2 Immune response ccl34b.8, tnfb Phagosome nfc1, nfc2
antigen processing and presentation cd74a, cd74b Lysosome ctss2.2, ctsba, tcirg1b
Cellular response to nitrogen starvation map1lc3b, gabarapl2, gabarapb NOD-like receptor signaling pathway il1b, inflammasome related gene
(jun, txnipa)
atp metabolic process pgam1a, pkma Glycolysis/gluconeogenesis pkma, pgam1a, aldocb
C-type lectin receptor signaling pathway egr3, il1b, irf1b
Mitophagy gabarapl2, atf4a, gabarapb
Salmonella infection il1b, tlr5b, fosab