Skip to main content
. Author manuscript; available in PMC: 2023 Aug 9.
Published in final edited form as: J Am Chem Soc. 2022 Jun 27;144(27):12184–12191. doi: 10.1021/jacs.2c03025

Figure 5.

Figure 5.

Telescoped reactions with additional chemical feedstocks. DIH = 1,3-diiodo-5,5’-dimethylhydantoin. The on-DNA reaction was performed on 10 nmol scale of HP-1 (2 mM in H2O, 1.0 equiv), 4CzIPN (1 mM in DMSO, 0.5 equiv), Na2CO3 (400 mM in H2O, 100 equiv), rt, 5 min, blue Kessil. a) 25 equiv of alkyl iodide (25 mM in DMSO) and 40 equiv of (TMS)3SiOH (40 mM in DMSO); b) 20 equiv of alkyl iodide (20 mM in DMSO) and 40 equiv of (TMS)3SiOH (40 mM in DMSO); c) 25 equiv of alkyl iodide (25 mM in DMSO) and 50 equiv of (TMS)3SiOH (50 mM in DMSO); d) 40 equiv of alkyl iodide (40 mM in DMSO) and 40 equiv of (TMS)3SiOH (40 mM in DMSO); e) off-DNA: 3 equiv of [(PhO)3PMe]I, overnight. On-DNA: 40 equiv of alkyl iodide (40 mM in DMSO) and 40 equiv of (TMS)3SiOH (40 mM in DMSO).