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. 2023 Jul 19;620(7973):366–373. doi: 10.1038/s41586-023-06357-1

Fig. 4. Sst44 cells are coupled through gap junctions.

Fig. 4

a, Patch-clamp electrophysiology analysis of pairs of Sst44 cells in brain slices. b, The mean membrane voltage (Vm) from an example connected pair. c, Expanded view of b to display the delay. The Vm for cells 1 and 2 are offset for visualization. d, The delay in the Vm of the follower cell for strong connections (Methods). Mean: 1.2 ms, 5 connections. e, Connection strength (∆Vm in the follower cell/∆Vm in the driver cell) from all pairs. A total of 14 out of 26 pairs (54%) were connected. Statistical analysis was performed using Wilcoxon signed-rank tests with Benjamini–Hochberg correction; P < 0.01. f, The connection strength for connected pairs with similar series resistance (<30% difference), showing that the connection strength is reciprocal. g, The mean Vm for the pair in b after adding inhibitors for NMDA (D-AP5), AMPA (NBQX) and GABAA (gabazine) receptors. h, The connection strength before and after adding synaptic blockers.