Extended Data Fig. 5. Pre/post biopsies scRNAseq identify various immune cell types.
a, Validation of the cluster annotation with dot plot showing top 5 genes markers that are enriched in clusters which were previously characterized by automatic cell annotation package. b, Tregs cluster identification with UMAP plot of sub-clustered lymphocyte showing Tregs expression markers: IL2RA, CTLA4, FOXP3 and CD4. c,d CD8 detection and quantification by IHC in mouse Pten driven tumors treated with control or NP137. c, representative image of IHC. Scale bar = 50 µm. d, Quantification of CD8 positive cells in control tumors (n = 4) vs NP137 treated (n = 8). Bars are mean values mean ± s.e.m., * P = 0.0162 by Mann-Whitney two-sided. e, Analysis of M2-macrophages, we used CD163 and MRC1 as markers on the UMAP plot of sub-clustered macrophages with the 2 integrated samples. f, The 2 panels show the M2 cell type number and the proportion of M2 in each sample compared to the total macrophage number.