Fig. 2. cGAS–STING activity drives degenerative processes in the aged brain.
a–c, Representative images and quantification of hippocampal IBA1+ cells (a), NeuN+ cells (b) and synaptophysin intensity (c) in the CA1 region from brain sections of young (n = 4) and aged mice (n = 8) that were treated or not with H-151. Scale bars, 200 μm (a and b (left)), 50 μm (a and b (right) and c). P = 3 × 10−5. d, Western blot analysis of pTBK1 in the brain lysates of young mice (n = 2), aged mice (n = 2) and aged mice acutely treated with H-151 (daily for 5 consecutive days, n = 3). e, cGAMP production measured by enzyme-linked immunosorbent assay (ELISA) in brain lysates of young and aged mice (n = 9). Data are mean ± s.e.m. P values were calculated using ordinary one-way ANOVA followed by Tukey’s multiple-comparison tests (a–c) or two-sided unpaired Student’s t-tests (e).