Fig. 3.

Alignment of the human post-mortem and hiPSC c9orf72 cell line proteomics dataset reveals synaptic vesicular dysfunction. a C9orf72 hiSC cell line ALS^C9orf72 shows 89% overlap with the human post-mortem dataset. b Heatmap demonstrates ALS group-specific protein changes using the 2501 congruent proteins. c-e Aligned dataset were used for further enrichment investigation. Based on fold enrichment and gene counts (dot size) top 10 KEGG pathways (c) were visualized by colorized dot plots. Different Gene Ontology (GO) terms (d-e) plotted by fold enrichment and the bar graph is colorized by gene number/pathway. f Synaptic enrichment analysis was created using SynGO database, revealing enriched synaptic functional terms such as synaptic vesicle cycle, presynaptic endocytosis and postsynaptic density. g Heatmap shows specific protein changes in terms of synaptic vesicle release. h Protein–protein interaction network built on the violet cluster identified in g