FIG. 3.

C-terminal truncation of the A17L protein in the presence of rifampin. Replicate wells containing BS-C-1 cells were infected with vaccinia virus and incubated at 37°C in the absence (A) or continued presence (B) of rifampin. At 6 h after infection, the cells were incubated for 30 min with [³⁵S]methionine. The cells were then washed and incubated with medium containing unlabeled amino acids. Cells were harvested after the 30-min pulse (time zero) and after 0.5, 1, 2, and 3 h of chase as indicated, lysed, and incubated with A17L C antibody, A17L N antibody, or antibody to the 4b core protein. The bound proteins were analyzed by SDS-PAGE and autoradiography. The positions and masses of marker proteins are indicated.