FIG. 4.

C-terminal truncation of the A17L protein failed to occur in cells infected at the nonpermissive temperature with an F10L mutant. Replicate BS-C-1 cells were infected with ts15 at 32°C (A) or 39.5°C (B). At 6 h after infection, the cells were incubated for 30 min with [³⁵S]methionine. The cells were then washed and incubated with medium containing unlabeled amino acids. Care was taken to maintain the temperature at 32 or 39.5°C during the labeling and chase periods. Cells were harvested after the 30-min pulse (time zero) and after 0.5, 1, 2, and 3 h of chase as indicated, lysed, and incubated with A17L C antibody or A17L N antibody. The bound proteins were analyzed by SDS-PAGE and autoradiography. The positions and masses of marker proteins are indicated.