FIG. 6.
Phosphorylation of the A17L and the A14L proteins. BS-C-1 cells were infected with vaccinia virus vA17LΔ5 at 37°C in the presence (+) or absence (−) of IPTG or with vaccinia virus ts15 virus at the permissive (32°C) or nonpermissive (39.5°C) temperature (T) and then labeled overnight with [35S]methionine (A) or with 32Pi (B). Immunoprecipitation was performed with A17L N antibody (A17L) or with A14L C antibody (A14L) as indicated. The bound proteins were analyzed by SDS-PAGE and autoradiography. U, uninfected cells. Positions and masses of marker proteins are shown on the right.