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Phosphorylation of the A17L protein in cells transfected with plasmids expressing the A17L and F10L genes. BS-C-1 cells were infected with vTF7-3 in the absence or presence of AraC and transfected with pVOTE.1-A17L (A17L) alone or together with.pVOTE.2-F10L (F10L). The cells were metabolically labeled with [35S]methionine (A) or 32Pi (B), and the lysates were immunoprecipitated with A17L N antibody and analyzed by SDS-PAGE and autoradiography.
