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. 2023 Aug 9;9:290. doi: 10.1038/s41420-023-01588-7

Fig. 6. Neuronal survival and immune cell infiltrations are not influenced by 11C7.

Fig. 6

A, B Spinal cord sections were immunofluorescently stained for myelin basic protein (MBP) and beta 3 tubulin (B3Tub) to visualize myelin and neuronal structures in the white and gray matters respectively. Extended myelin damage was observed in the white matter of EAE mice treated with control IgG (A), although 11C7 treatment was associated with modest myelin damage (B). C The density of large B3Tub-positive neurons was quantified to determine if 11C7 protected motoneurons in the ventral gray matter. Quantitatively, the number of neurons did not differ between the 2 experimental groups. D–I T cell and monocyte infiltration was observed in the lumbar spinal cord of EAE mice using CD3 and Mac3 as specific markers, respectively. The density of CD3+ (F) and Mac3+ (I) cells did not differ between control and 11C7 treatments. Each dot represents a different animal. Scale bars: A, B = 500 μm, close-ups= 100 μm; D, E, G, H = 200 μm.