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. 2023 Aug 9;14:4808. doi: 10.1038/s41467-023-40482-9

Fig. 10. Effect of Thr352Ala mutation in CXCR7 on βarr2 recruitment and endosomal localization.

Fig. 10

a–d Dose-response curves of CXCL12- and VUF11207-induced βarr2 recruitment to selected phosphorylation site mutants of CXCR7 in the Tango and NanoBiT assays (mean ± SEM; n = 6 independent experiments for a, b and n = 3 independent experiments for c, d; normalized with luminescence signal for WT at maximal ligand dose treated as 100%). e, f Dose-response curves of CXCL12- and VUF11207-induced βarr2 endosomal localization for the selected phosphorylation site mutants of CXCR7 in the NanoBiT assay (Receptor+SmBiT-βarr2 + LgBiT-FYVE) (mean ± SEM; n = 3–7 independent experiments; i.e., for CXCL12-induced βarr2 endosomal localization: WT, n = 6; T352A, n = 3; and Cluster2, n = 3; for VUF11207-induced βarr2 endosomal localization: WT, n = 7; T352A, n = 3; and Cluster2, n = 4; normalized with the luminescence signal at minimal ligand dose treated as 1). Source data are provided as a source data file.