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. 2023 Aug 9;14:4808. doi: 10.1038/s41467-023-40482-9

Fig. 3. Receptor sub-type selectivity of VUF11207 and its ability to promote cell migration.

Fig. 3

a, b VUF11207-induced βarr2 recruitment for all the CXC chemokine receptors (CXCR1-7) in the PRESTO-Tango and Tango assay, respectively (mean ± SEM); n = 5 independent experiments; normalized with the luminescence signal at minimal ligand dose treated as 1. c VUF11207-induced Gαi-coupling for all the CXC chemokine receptors (CXCR1-7) in the GloSensor assay (mean ± SEM; n = 3 independent experiments; normalized with luminescence signal at minimal ligand dose treated as 100%). d Migration of MDA-MB-231 stably expressing CXCR7 in response to indicated ligands. The experiment was carried out using a 2D microfluidic device and each point on the graph represents an individual cell (n = 600 cells examined over 2 independent experiments, One-way ANOVA, Šídák’s multiple comparisons test). The exact p-values are as follows: Control vs. CXCL12 (p < 0.0001), Control vs. VUF11207 (p < 0.0001), Control vs. CXCL12 + VUF11207 (p < 0.0001). The lower and upper whiskers represent the 5th percentile and the 95th percentile respectively and the bounds of box correspond to 25th and 75th percentile. The cross inside the box indicates the mean (Control: 220.798, CXCL12: 390.201, VUF11207: 341.980, CXCL12 + VUF11207: 325.656). The solid line at the 50th percentile indicates the median (Control: 111.150, CXCL12: 325.000, VUF11207: 291.850, CXCL12 + VUF11207: 257.07)5. (****p < 0.0001). Source data are provided as a source data file.