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. 2023 Aug 9;14:4808. doi: 10.1038/s41467-023-40482-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a, b CXCL12- and VUF11207-induced βarr2 recruitment, respectively, to the indicated phosphorylation site mutants of CXCR7 using the Tango assay (mean ± SEM); n = 4–6 independent experiments; i.e., for CXCL12: WT, S350A, T352A, S355A, S350A + T352A, T352A + S355A, and Cluster2, n = 6; S350A + S355A, n = 4; for VUF11207-induced βarr2 recruitment: WT, S350A, T352A, S355A, S350A + T352A, T352A + S355A, and Cluster2, n = 6; S350A + S355A, n = 4; normalized with luminescence signal at maximal ligand dose for wild-type treated as 100%, Two-way ANOVA, Dunnett’s multiple comparisons test. The exact p-values are: for CXCL12-induced βarr2 recruitment; WT vs. S350A (p < 0.0001), WT vs. T352A (p < 0.0001), WT vs. S355A (p < 0.0001), WT vs. S350A + T352A (p < 0.0001), WT vs. T352A + S355A (p < 0.0001), WT vs. S350A + S355A (p < 0.0001), WT vs. Cluster2 (p < 0.0001). For VUF11207; WT vs. S350A (p < 0.0001), WT vs. T352A (p < 0.0001), WT vs. S355A (p < 0.0001), WT vs. S350A + T352A (p < 0.0001), WT vs. T352A + S355A (p < 0.0001), WT vs. S350A + S355A (p < 0.0001), WT vs. Cluster2 (p < 0.0001). c, d CXCL12- and VUF11207-induced βarr2 recruitment, respectively, to the indicated phosphorylation site mutants of CXCR7 using the NanoBiT assay (mean ± SEM); n = 3 independent experiments; normalized with luminescence signal at maximal ligand dose for wild-type treated as 100%, Two-way ANOVA, Dunnett’s multiple comparisons test. The exact p-values are: for CXCL12; WT vs. S350A (p = 0.9994), WT vs. T352A (p < 0.0001), WT vs. S355A (p = 0.0001), WT vs. S350A + T352A (p < 0.0001), WT vs. T352A + S355A (p < 0.0001), WT vs. S350A + S355A (p < 0.0001), WT vs. Cluster2 (p < 0.0001). For VUF11207; WT vs. S350A (p = 0.8957), WT vs. T352A (p < 0.0001), WT vs. S355A (p < 0.0001), WT vs. S350A + T352A (p < 0.0001), WT vs. T352A + S355A (p < 0.0001), WT vs. S350A + S355A (p < 0.0001), WT vs. Cluster2 (p < 0.0001) (***p < 0.001, **** p < 0.0001, ns non-significant). Source data are provided as a source data file.