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. 2023 Aug 9;14:4812. doi: 10.1038/s41467-023-40536-y

Fig. 1. PF-07208254 improves cardiac function similar to BT2 and reduces pBCKDH in mice.

Fig. 1

A–J Mice were subjected to TAC surgery. A Study design. Mice were switched to chow containing PF-07208254 or BT2 48 h prior to surgery. Four weeks after surgery, echocardiography was performed. Five to six weeks after surgery, the animals were euthanized. B, C Echocardiography measurements B. % Fractional shortening (%FS) (**p = 0.007, *p = 0.015), C % Ejection fraction (%EF). D–E Tissue weights were measured at euthanasia. D Heart weight normalized to tibia length (*p = 0.018). E Lung weight normalized to tibia length. (N = 14–26 animals/group for B–E; statistics performed by one-way ANOVA with Tukey post-hoc test for B–D and pairwise Wilcoxon test was performed for E). F–H Heart tissue was immunoblotted. F Representative Western blot images of pBckdh, Bckdh, BDK and Gapdh. G-H Densitometry from F (N = 3–6 animals/group; statistics performed by pairwise Wilcoxon test. #; p < 0.0001). I, J Plasma BCAA and BCKA levels were quantified at day 38. I Leucine. J Ketoleucine (N = 10–15 animals/group; statistics performed by one-way ANOVA with Tukey post-hoc test. #p = 0.0001). Data represent the mean ± SEM. Source data are provided as a Source Data file.