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. 2023 Aug 9;9:292. doi: 10.1038/s41420-023-01579-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Immunofluorescent staining showing the number of LC3 punctas in the T24-mRFP-GFP-LC3 KD cells and their control cells. Cells were stained by indirect immunofluorescence using anti-LC3 antibody, and the number of LC3 puncta per cell was quantified. Scale bars = 100 μm. B Representative TEM ( × 20,000) images of T24 cells and quantification of autophagic vacuoles (autophagosomes and autolysosomes) in subsets of 10 randomly-selected cells of each type. Red arrows, autophagic vacuoles. C Western blotting assay showed that the expression of LC3B and Beclin1 decreased and P62 showed the opposite change with the knocking down of RAB14. *P < 0.05, **P < 0.001.