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. 2023 Aug 9;9:292. doi: 10.1038/s41420-023-01579-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Immunofluorescence staining of E-cadherin. T24 cells were treated with rapamycin (RAPA), which is a specific inhibitor of mTOR protein, to study the effect of autophagy. Immunofluorescent staining showed that shRAB14 + RAPA group showed similar levels of E-cadherin as shNC group, but it showed E-cadherin was down-regulated compared with shRAB14 group. B Immunofluorescence staining of Vimentin. Immunofluorescent staining showed that shRAB14 + RAPA group showed similar levels of vimentin as shNC group, but vimentin was significantly upregulated compared with shRAB14 group. C Representative TEM ( × 20,000) images of T24 cells and quantification of autophagic vacuoles (autophagosomes and autolysosomes) in subsets of 10 randomly-selected cells of each type. D Western blotting assay of E-cadherin, N-cadherin and Vimentin. E Cell migration and invasion were ananlyzed. *P < 0.05, **P < 0.001.