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. 2023 Aug 9;13:12888. doi: 10.1038/s41598-023-40051-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Genes associated with the “Development of the retina” were differentially altered by 30 mGy and 180 mGy. Network of genes altered as determined from the list of altered genes in “Development of retina” (Supplemental Table S8) and other genes selected on day 35. Genes with fold changes > 1.2 were increased (red), and those < − 1.2 were decreased (purple). Predicted activation (orange) and predicted inactivation (blue) of genes without their own activation/inactivation were calculated by IPA with altered expression of related genes. Genes with predicted relationships are connected by lines. Top: 30 mGy. Left bottom. Bottom: 180 mGy. Each dose sample for single RNA-seq included 6 to 12 EBs. RNA-seq was repeated three times for each dose.