Fig. 8.

Cell clustering and MAPT expression across cell types in control and PSP samples. a, b Unbiased clustering of snRNAseq data in control (a) and PSP (b) cases. UMAP plots of cell clusters with each cell group are coloured individually. The colours of the cell labels in (a) also correspond to (b), and the cell clusters identifies in (a) also correspond to the cell clusters in (c, d). UMAP projection of 31,000 brain cells included, where each cell is grouped into one of the 10 cellular clusters (distinguished by their colours). Cell types were annotated according to expression of known marker genes; cluster name is indicated in the legend. Oligodendrocytes (red) comprised the largest cell cluster. c, d MAPT expression (purple) in controls (c) and PSP (d) cases in each cell cluster represented in UMAP plots from panels (a) and (b). e MAPT expression in controls (blue) and PSP (red) cases. MAPT expression was significantly lower in inhibitory neurons, astrocytes and oligodendrocyte precursor cells (OPCs) in PSP cases compared to controls (P < 0.0001 for all, n = 3). Mature oligodendrocytes and excitatory neurons contained similar levels of MAPT gene expression in controls and PSP