Fig. 4.

Competitive binding of target modules to UniCAR-NK-92 cells. NK-92/5B9.z (a) and NK-92/5B9.28.z cells (b) were incubated with 9.52 nM of APC-labeled 5FE protein in the presence of increasing concentrations of unlabeled TM proteins as indicated, washed, and remaining bound APC-5FE was determined by flow cytometry. A PD-L1-specific IgG4 Fc fusion protein served as control. Mean values ± SD from 3 individual experiments and interpolated sigmoidal curves are shown