FIG. 2.
env V3 genotype of provirus in uncultured PBMCs (A) and of virus isolates from the NH family (B). Deduced amino acids of the V3 sequences were aligned with a consensus sequence made from 21 subtype E NSI isolates (12, 27). The open box indicates a potential site for N-linked glycosylation. The shaded box indicates a subgroup of V3 sequences that are characterized by the presence of a GPGR motif at the tip of the V3 loop and basic substitutions with respect to the subtype E NSI consensus. Net charge is the number of positively charged amino acids (R and K) minus the number of negatively charged amino acids (D and E). (A) Sequences from uncultured PBMCs of NH1, NH2, and NH3 in June 1993 and of NH2 in March 1996 (NH2-II) and January 1997 (NH2-III). The arrows indicate a sequence identical among the three family members. A shaded arrowhead indicates a sequence similar to those from NH1 isolates. (B) Sequences from HIV-1 isolates from NH1 (HIV-1NH1), NH2 (HIV-1NH2), and NH3 (HIV-1NH3) in June 1993. The shaded arrowheads indicate sequences identical or similar to those from uncultured PBMCs of the corresponding individuals.