Figure 1.

PS-ASO colocalizes with Stabilin receptor after Stabilin-mediated endocytosis

(A) 190-HARE (Stabilin-2), (B) Stabilin-1, and (E) EV cell lines, respectively, were incubated with 0.1 μM PS-ASO for 30 min (pulse), followed by incubation with no PS-ASO media for variable times (0, 30, 60, 90, and 120 min) (chase). Immunocytochemistry was performed and mounted slides were imaged by confocal microscopy. Images were processed with EzColocalization, an ImageJ plugin. Correlation quantification (Pearson correlation coefficient) between (C) Stabilin-2 and PS-ASO and (D) Stabilin-1 and PS-ASO (n = 6 images). PS-ASOs are not internalized in EV cell lines. Some material was adhered on the cell surface, giving blue florescence.