Figure 6.

Chloroquine rescues the reduced efficacy of PS-ASO caused by EEA1/Rab5C/Rab7A knockdown (KD)

190-HARE cells were treated with (A) EEA1 siRNA, (B) Rab5C siRNA, and (C) Rab7A siRNA, respectively, for 48 h. PS-ASO and chloroquine treatment was done as explained for Figure 5C. Malat1 mRNA expression was quantified with qRT-PCR. Statistical analysis was performed using Student’s t test. Data presented as mean ± SEM. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001. n ≥ 3 in triplicate.