Fig. 3.

Goldner's trichrome (A-C & G-I), HE (E-H), and TRAcP activity stained bone sections. Goldner's trichrome-stained sections illustrate the trabecular osteoid perimeters (A-C) with an adjacent HE-stained section illustrating the absence of osteoblasts with a clear cuboidal morphology above osteoid surfaces (D-F). Moreover, eroded surfaces are illustrated on a Goldner's trichome stained section, with a broken lamella structure (G-E), while a TRAcP activity stained adjacent section illustrates the absence of osteoclasts (J-L). Finally, a positive TRAcP activity stain was included, where osteoclasts are visualized. White dashed lines highlight osteoid surfaces, red dashed lines highlight eroded surfaces and red arrows highlight osteoclasts.