Figure 2.

Phenotypic and metabolic consequences of PRPP decrease in yeast. A–E, Wild-type (Y12325), prs1Δ prs3Δ (Y12562), and prs2Δ prs4Δ prs5Δ (Y12649) were exponentially grown in SDcasaWAU medium for 24 h before measurement of biomass production rate (C), median volume (D), population doubling time (E), or metabolic extraction, separation and quantification (A and B), done as described in Experimental procedures. [AXP] = [ATP] + [ADP]+ [AMP] (individually presented in Fig. S1, A, E, and I). Each parameter was determined on four to eight independent measurements. Numbers on each panels correspond to p-values calculated from a Welch’s unpaired t test.