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. 2023 Jul 4;299(8):105011. doi: 10.1016/j.jbc.2023.105011

Figure 4.

Figure 4

Metabolic and phenotypic consequences of PRS1-PRS3 overexpression. Wild-type (Y11451) strain was transformed with either the empty vectors (vectors, YepLac181 and YepLac195) or plasmids allowing overexpression of PRS1 (p6160) and PRPS3 (p5671) (PRS1 + PRS3). Transformants selected on SC -Ura -Leu medium were exponentially grown for 24 h in the same liquid medium before extractions and quantification of metabolites from eight independent cultures (A–H) or determination of either the population doubling time (I), the relative biomass production rate (J) or the median volume (K). The p-values, calculated from Welch’s unpaired t test are presented by numbers.