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. 2023 Jul 4;299(8):105011. doi: 10.1016/j.jbc.2023.105011

Figure 5.

Figure 5

PRPP content is limiting for yeast proliferation and nucleotide synthesis.A, the growth defect of the prs1 prs3 mutant is restored by expression of either the human or bacterial PRPP synthetases. Wild-type (Y11418) and prs1Δ prs3Δ (Y11983) strains were transformed with either an empty vector (None) or plasmids allowing expression of human (PRPS1 (p6001) or PRPS2 (p6020)) or bacterial (PrsA; p5909) PRPP-synthetases. Transformants were selected on SDcasaWA medium and serial dilutions (1/10) of transformants were spotted on SDcasaWA and plates were imaged after 36 h (PrsA) or 48 h (PRPS) at 30 °C. BI, transformants were exponentially grown for 24 h in liquid SDcasaWA before determination of the population doubling time (B) or extractions and quantification of metabolites (CH). Each metabolite was quantified from six independent cultures. The p-values, calculated from Welch’s unpaired t test, are presented by numbers. Overexp., overexpressed.

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