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. 2023 Jul 4;299(8):105011. doi: 10.1016/j.jbc.2023.105011

Figure 6.

Figure 6

Growth and metabolic defects of the prs1 prs3 mutant are rescued by supplementation with PRPP-not-requiring precursors.A, addition of nucleoside precursors partially restores the prs1 prs3 mutant growth defect. Wild-type (Y11418) and prs1Δ prs3Δ (Y11985) strains were transformed with the hENT1 expressing plasmid (p4991). Transformants were selected on SDcasaW medium supplemented with adenosine (Ado, 300 μM), Uridine (Uri, 300 μM), Tryptophan (300 μM), and Nicotinamide mononucleotide (NMN, 100 μM). Transformants were serial diluted (1/10) and spotted on SDcasaW supplemented or not with Ado, Uri, Trp, and NMN. Plates were imaged after 36 h at 30 °C. B-H, transformants were exponentially grown for 24 h in SDcasaW liquid medium supplemented or not with the indicated precursors prior to population doubling time measurement (B) or metabolic extraction, separation, and quantification (C–H). The p values, calculated from a Welch’s unpaired t test, are presented by either blue (comparison with wild-type cells grown without precursors) or green numbers (comparison with prs1Δ prs3Δ cells grown without precursors).