Figure 3.

Regioselective hydroxylation of racemic OMP by CYP102A1 variants. Hydroxylation of racemic OMP was performed as described in Experimental procedures, and metabolites were analyzed by LC-MS/MS to confirm identities of major metabolites. A, elution of authentic OMP, 5OH, and sulfone metabolites; (B) elution of OMP metabolites by HLM; (C) elution of OMP metabolites by UD3; (D) MS² spectrum of the major metabolite produced by UD3 at 12.7 min in C; (E) assignments of MS2 peaks observed in D; (F) steady-state turnover rates for production of 5OH metabolite. 5OH, 5-hydroxyomeprazole; HLM, human liver microsome; LC-MS/MS, liquid chromatography-tandem mass spectrometry; OMP, omeprazole.