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Stereoselective hydroxylation of OMP enantiomers by CYP102A1 variants.A–C, represent HPLC elution profiles of metabolites produced by UD1, UD2, and UD3, respectively, in the presence of either (R)-OMP (black trace) or (S)-OMP (red trace). D, steady-state turnover rates for the 5OH metabolite. The rates of DM and TM are included for comparison. 5OH, 5-hydroxyomeprazole; DM, double mutant; OMP, omeprazole; TM, triple mutant.
