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Rates of NADPH oxidation by CYP102A1 variants
| Variant | kcat (min−1) | KM (μM) | kcat/KM (μM−1 min−1) | Coupling (%) |
|---|---|---|---|---|
| DM (A82F/F87V) | 1306.0 ± 45.3 | 28.8 ± 6.8 | 45.3 | 3.0 |
| TM (DM/L188Q) | 943.1 ± 26.2 | 24.4 ± 4.8 | 38.6 | 5.2 |
| UD1 (TM/L75I) | 662.2 ± 28.1 | 86.6 ± 11.8 | 7.6 | 6.5 |
| UD2 (TM/A264G) | 1276.0 ± 96.8 | 131.3 ± 26.7 | 9.7 | 5.5 |
| UD3 (TM/A328V) | 1144.0 ± 57.2 | 59.5 ± 15.8 | 19.2 | 5.9 |
The rates were determined in 1× PBS solution at 25 °C as described in Experimental procedures.
