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Persistant alterations in epigenetic landscape of differentiated neurons after developemental Pb exposure. Image panel of neurons at (A) Day 25 and (B) Day 45 stained for 5 mC, H3K4me3, and H3K27me3 markers. Scale bar = 10 μm. Relative change in integrated intensity of 5 mC, H3K4me3, and H3K27me3 markers of neurons at (C) Day 25 and (D) Day 45. Data = Mean ± SE. N > 600 cells from six independent differentiations. N.S., not significant; ∗p < 0.05 and ∗∗∗p < 0.001.
