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. 2023 Jul 26;8(31):28090–28097. doi: 10.1021/acsomega.3c00602

Table 2. Contents of Bioactive Composition in the TFE by UHPLC-ESI-QqQ-MS/MS.

composition RT (min) linear equation correlation coefficient: R2 concentration (ppm)
caffeine 3.627 y = 48,366,330x + 2,049,601 0.9990512 4.33 ± 0.64
caffeic acid 4.574 y = 12,402,100x + 159497.3 0.9997329 0.06 ± 0.01
protocatechuic acid 2.670 y = 7007.355x + 6762.765 0.8132233 82.52 ± 2.98
gallic acid 1.640 y = 3,183,944x + 36054.58 0.9996324 1.58 ± 0.05
p-coumaric acid 5.535 y = 6,349,098x + 44423.86 0.9995383 0.04 ± 0.01
ferulic acid 5.706 y = 388346.4x + 13558.94 0.9996573 0.16 ± 0.01
catechin 3.967 y = 2,135,389x + 2950.069 0.9994077 0.05 ± 0.01
epicatechin (EC) 4.763 y = 1,426,047x + 24115.47 0.9996847 0.14 ± 0.02
gallocatechin (GC) 2.386 y = 1,182,815x + 25075.34 0.9997533 0.12 ± 0.01
epigallocatechin (EGC) 3.494 y = 2,307,225x + 3103.182 0.9997687 0.16 ± 0.02
catechin gallate (CG) 5.647 y = 3,066,125x + 11638.07 0.9995410 1.75 ± 0.02
gallocatechin gallate (GCG) 5.067 y = 7,645,719x – 68883.98 0.9988892 0.02 ± 0.00
epigallocatechin gallate (EGCG) 5.065 y = 3,942,501x – 39618.69 0.9994777 2.59 ± 0.02