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. 2023 May 31;9(22):eadg3365. doi: 10.1126/sciadv.adg3365

Fig. 1. Schematic illustration of the locoregional generation of S. aureus–specific super CAR-MΦs at the bone-implant interface for preventing PJI.

Fig. 1.

(A) Chemical structure of the peptide-SA monomer. (B) Diagram of anti-SasA CAR and CASP11 short hairpin RNA (shRNA) structure in plasmid DNA (pDNA). CMV, cytomegalovirus; EGFP, enhanced green fluorescent protein. (C) Schematic illustration of the preparation of pDNA-laden peptide nanoparticle (pPNP). (D) Schematic illustration of the pPNP coating on an implant (Ti-pPNP). HS, heparan sulfate. (E) pPNP coating generates S. aureus–specific super CAR-MΦs for tracking and eradicating S. aureus infection; this approach orchestrates periprosthetic anti-infection and osseointegration in an arthroplasty mouse model. BMSC, bone mesenchymal stem cell; siRNA, small interfering RNA.