FIG. 6.

CD4⁺ CTL clones lyse HepG2 bystander target cells via a Fas/FasL-mediated mechanism. Clones were incubated with either CMA at 10 nM or BFA at 10 μM for 2 h prior to the addition of target cells. Lysis of labeled HepG2 bystander targets in the presence of unlabeled peptide-presenting BLCL was assessed after an 8-h incubation. The E/T ratio was 10:1. The spontaneous release was ≤27% for all conditions.