Fig. 1.

Mutant p53 controls ITGA5 expression via ENTPD5. a-b Western blots of pancreatic ductal adenocarcinoma cell lines (MIA PaCa-2, Panc-1) for expression of p53, ENTPD5, and ITGA5 following transfection with siRNAs targeting p53, ENTPD5 or ITGA5 as indicated. c Western blot for p53, ITGA5, and ENTPD5 following p53-depletion in MIA PaCa-2 cells with doxycycline-inducible ENTPD5 expression. Doxycycline was added to the media 24 h before siRNA transfection. d Western blot for p53, ITGA5, and ENTPD5 following p53 depletion in MIA PaCa-2 cells with doxycycline-inducible expression of p53^R248W or p53^R175H mutants. Doxycycline was added 24 h before transfection with an siRNA targeting the 3’UTR of the endogenous mutp53. In all Western blots, ITGA5 levels were quantified by ImageJ and normalized to the nsi control. β-actin served as a loading control. Mock: non-transfected cells; nsi: non-targeting control siRNA