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. 2023 Aug 10;42:203. doi: 10.1186/s13046-023-02785-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CANX/CALR chaperones are essential for mutant p53-dependent ITGA5 activity. a Western blot of MIA PaCa-2 cells following siRNA-mediated depletion of CANX, CALR, UGGT, p53, ITGA5, or ITGB1. b-c Cell-spreading assay. MIA PaCa-2 cells were depleted of indicated proteins and analyzed by spinning disc microscopy 45 min after plating on FN-coated slides: b percentage of spread cells shown as mean ± SD (n = 3 transfections with 125–200 counted cells per transfection); c representative images. d-f FN-mediated adhesion, migration, and invasion of MIA PaCa-2 cells depleted of CANX, CALR, or UGGT. The analysis was performed as described in Fig. 2. Results are shown as mean ± SD (n = 3 independent experiments). Statistical significance was tested using two-way (d) or one-way (e–f) ANOVA followed by Dunnett’s multiple comparisons test: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant. Mock: non-transfected cells; nsi: non-targeting control siRNA