Fig. 5.

α-glucosidase inhibitors block mutant p53-dependent ITGA5 expression and function. a-b Western blot for p53, ENTPD5, and ITGA5 of MIA PaCa-2 cells treated with the indicated doses of the α-glucosidase inhibitors Acarbose and UV-4. c-d Adhesion kinetics on FN of Acarbose- and UV-4-treated MIA PaCa-2 cells. e–g FN-dependent transwell migration and invasion of MIA PaCa-2 cells treated with Acarbose and UV-4 as indicated. h Representative images of crystal violet-stained transwell inserts (invasion) and proliferation controls, documenting comparable cell seeding and viability. All graphs show the mean ± SD of n = 3 biological replicates. Statistical significance was tested using two-way ANOVA followed by Dunnett’s multiple comparisons test: ***, p < 0.001; ****, p < 0.0001; ns, not significant