Fig. 6.

Degradation of mutant p53 blocks ITGA5-mediated cancer cell motility in vitro. MIA PaCa-2 pIND-ENTPD5 cells (with tet-inducible expression of ENTPD5) were induced with doxycycline (+ tet) as indicated and treated with Ganetespib (Ganet, 0.5–1.0 µM), SAHA/17-AAG (10–15 µM) or vehicle. a Western blot for p53, ENTPD5 and ITGA5. β-actin is shown as a loading control. b Adhesion kinetics on FN. c FN-mediated transwell migration and invasion. All graphs show the mean ± SD of n = 3 biological replicates. Statistical significance was tested using two-way ANOVA followed by Dunnett’s multiple comparisons test: ****, p < 0.0001; ns, not significant