Table 1.
Overview of preclinical studies based on CAR-NK cell therapy for hematological malignancies
| Disease | Target | NK cell source | Intracellular domain | Transduction methods | References |
|---|---|---|---|---|---|
| B-cell malignancies | CD19 | PB-NK | CD28.CD3ζ | Retrovirus | [141] |
| B-cell malignancies | CD19 | PB-NK | 4-1BB.CD3ζ | Retrovirus | [142] |
| B-cell malignancies | CD19 | CB-NK | CD28.CD3ζ+IL-15 | Retrovirus | [128, 143] |
| B-cell malignancies | CD19; CD20 | NK-92 | CD3ζ | Retrovirus | [144] |
| B-cell malignancies; acute myeloid leukemia | CD19; CD276 | NK-92 | CD28.CD3ζ | Retrovirus | [145] |
| B-cell lymphoma; multiple myeloma | CD19; BCMA | NK-92 | 4-1BB.CD3ζ | Electroporation | [76] |
| B-cell acute lymphoblastic leukemia | FLT3 | NK-92 | CD28.CD3ζ | Lentivirus | [146] |
| B-cell malignancies | CD20 | NK-92 | CD3ζ | Retrovirus | [147] |
| B-cell non-Hodgkin lymphoma | CD20 | PB-NK | 4-1BB.CD3ζ | Electroporation | [148, 149] |
| T-cell malignancies | CD3 | NK-92 | 4-1BB.CD28.CD3ζ | Lentivirus | [150] |
| B-cell malignancies T-cell malignancies | CD4 | NK-92 | CD28.4-1BB.CD3ζ | Lentivirus | [151] |
| T-cell malignancies | CD3; CD5 | NK-92 | 2B4.CD3ζ/CD28.CD3ζ | Retrovirus | [152] |
| T-cell malignancies | CD5 | NK-92 | 4-1BB.CD28.CD3ζ | Lentivirus | [153] |
| T-cell malignancies | CD5 | NK-92 | CD28.CD3ζ | Lentivirus | [154] |
| T-cell malignancies | CD5 | NK-92 | 4-1BB.CD3ζ/2B4.CD3ζ | Lentivirus | [155] |
| T-cell leukemia | CD7 | NK-92MI | CD28.4-1BB.CD3ζ | Electroporation | [156] |
| EBVA3C+T-cell lymphoblastic cells | EBNA3C | NK-92MI | 4-1BB.CD3ζ | Retrovirus | [157] |
| Multiple myeloma | CD138 | NK-92MI | CD3ζ | Lentivirus | [158] |
| Multiple myeloma | CD138; CD19 | NK-92 | CD28.4-1BB.CD3ζ | Lentivirus | [159] |
| Multiple myeloma | CS1 | NK-92 | CD28.CD3ζ | Lentivirus | [160] |
| Multiple myeloma | NKG2D | PB-NK | 4-1BB.CD3ζ | Lentivirus | [161] |
| Acute myeloid leukemia | NKG2D | PB-NK | CD28.4-1BB.CD3ζ+IL-15 | Electroporation | [162] |
| Acute myeloid leukemia | CD33 | PB-NK | 4-1BB.CD3ζ | Lentivirus | [163] |
| Acute myeloid leukemia | CD38 | KHYG-1; PB-NK | CD28.CD3ζ | Electroporation | [164] |
| Acute myeloid leukemia | CD123 | PB-NK | CD28.4-1BB.CD3ζ | Retrovirus | [165] |
| Acute myeloid leukemia | CD123 | PB-NK | 4-1BB.CD3ζ | Retrovirus | [166] |
| Acute myeloid leukemia | CD123 | NK-92 | 4-1BB.CD28.CD3ζ | Retrovirus | [167] |
| Acute myeloid leukemia | NPM1c | PB-NK | 4-1BB.CD3ζ | Lentivirus | [168] |