Table 2.
Overview of preclinical studies based on CAR-NK cell therapy for solid tumors
| Disease | Target | NK cell source | Intracellular domain | Transduction methods | References |
|---|---|---|---|---|---|
| Glioblastoma | EGFR; EGFRvIII | NK-92; NKL | CD28.CD3ζ | Lentivirus | [81, 218] |
| Glioblastoma | EGFR; EGFRvIII | PB-NK | CD28.CD3ζ | Retrovirus | [205] |
| Glioblastoma | Her2 | NK-92 | CD28.CD3ζ | Lentivirus | [192] |
| Glioblastoma | GD2; NKG2D | NK-92 | CD28.CD3ζ; DAP10.CD3ζ | Electroporation | [219] |
| Glioblastoma | B7H3 | CB-NK | CD28.CD3ζ | Retrovirus | [220] |
| Breast cancer | B7H6 | NK-92 | NKp30. CD3ζ | Retrovirus | [221] |
| Breast cancer | Epcam | NK-92 | CD28.CD3ζ+IL-15 | Lentivirus | [222] |
| Breast cancer | Her2 | NK-92 | CD28.CD3ζ | Electroporation | [223] |
| Breast cancer | Her2 | PB-NK | CD28.CD3ζ | Lentivirus | [194] |
| Breast cancer | EGFR | NK-92; PB-NK | CD28.CD3ζ | Lentivirus | [224] |
| Ovarian cancer | GPC3 | iPSC-NK | CD28.4-1BB.CD3ζ | Lentivirus | [225] |
| Ovarian cancer | MSLN | NK-92 | 2B4.CD3ζ | Lentivirus | [207] |
| Ovarian cancer | MSLN | iPSC-NK; NK-92 | 2B4.CD3ζ | Lentivirus | [96] |
| Ovarian cancer | CD133 | NK-92 | CD28.4-1BB.CD3ζ | Lentivirus | [209] |
| Ovarian cancer | CD24; MSLN | NK-92 | CD28.4-1BB.CD3ζ | Lentivirus | [210] |
| Ovarian cancer | CD44 | NK-92 | CD28.4-1BB.CD3ζ | Lentivirus | [226] |
| Ovarian cancer | FRα | NK-92 | CD28.4-1BB.CD3ζ | Lentivirus | [208] |
| Prostate cancer | PSMA | NK92MI | NKG2D | Lentivirus | [227] |
| Prostate cancer | PSMA | NK-92 | 2B4.CD3ζ | Lentivirus | [228] |
| Prostate cancer | PSMA | NK-92 | CD28.CD3ζ | Lentivirus | [229] |
| Pancreatic cancer | MSLN | NK-92 | 4-1BB.CD3ζ | Unknown | [230] |
| Pancreatic cancer | PSCA | PB-NK | CD28.CD3ζ | Retrovirus | [231] |
| Pancreatic cancer | FRα | NK-92 | CD27.CD3ζ | Lentivirus | [212] |
| Colorectal cancer | CEA | NK-92MI | CD3ζ | Retrovirus | [217] |
| Colorectal cancer | EPCAM; EGFRvIII; Fzd | NK-92 | CD28.CD3ζ | Lentivirus | [232] |
| Colorectal cancer | EPCAM | NK-92 | 4-1BB.CD3ζ | Lentivirus | [233] |
| Colorectal cancer | NKG2D | NK-92 | DAP12; CD3ζ | Electroporation | [139] |
| Gastric cancer | MSLN | NK-92 | 2B4.CD3ζ | Lentivirus | [77] |
| Small cell lung cancer | DLL3 | NK-92 | 2B4.CD3ζ | Lentivirus | [234] |
| non-small cell lung cancer | CD70 | PB-NK | CD28.4-1BB.CD3ζ | Retrovirus | [235] |
| Lung adenocarcinoma | c-Met | NK-92 | 2B4.DAP10.CD3ζ | Lentivirus | [137] |
| Neuroblastoma | B7-H3 | NK-92 | CD28.CD3ζ | Lentivirus | [236] |
| Melanoma | B7-H3 | NK-92 | CD28.CD3ζ | Lentivirus | [237] |
| Multiple solid tumors | HLA-G | PB-NK | DAP12+iC9 | Lentivirus | [238] |
| PSCA+ cancer | PSCA | YTS; PB-NK | DAP12 | Lentivirus | [135] |
| Neuroblastoma | GD2 | NK-92 | CD3ζ | Retrovirus | [239] |
| Ewing sarcoma | GD2 | PB-NK | 4-1BB.CD3ζ | Retrovirus | [240] |
| Hepatocellular Cancer Cells | GPC3 | NK-92 | DNAM1.2B4.CD3ζ | Lentivirus | [241] |
| Hepatocellular Cancer Cells | GPC3 | NK-92 | CD28.CD3ζ | Lentivirus | [215] |