Figure 2.

Model simulations and fittings to the OCR experimental data of isolated mitochondria from the heart and kidney cortex and OM mitochondria in response to sequential and incremental ADP additions. Comparison of the time courses of mitochondrial OCR representative data (A–C) with the corresponding model simulations (D–F) in the heart, kidney cortex, and kidney OM in response to sequential ADP additions of 25, 50, 75, 100, 150, and 250 µm. Model fitting to the peaks of the OCR data after each ADP addition (G–I). Five different substrate combinations are used including PM (5:2.5 m m), AM (5:2.5 m m), GM (5:2.5 m m), SUC (10 m m), and SUC + ROT (10 m m + 0.5 µm). The representative data are shown with dashes, the OCR peaks data are shown with symbols, and the model simulations are shown with solid lines. Model simulations for SUC and SUC + ROT overlap in the kidney cortex and OM mitochondria (pink and cyan lines).