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. 2023 Aug 10;13(8):e1352. doi: 10.1002/ctm2.1352

FIGURE 5.

FIGURE 5

AF9 positively regulates the expressions of phosphoenolpyruvate carboxykinase 2 (PCK2) and FBP1, then hampers glycolysis. (A) RNA‐seq analysis of HT29 cells with or without depleting AF9 or HCT116 cells with or without forced expression of AF9. (B) AF9 positively regulated genes were found in HT29 and HCT116 cells. (C) The name and ratio value of TOP10 genes positively regulated by AF9 were listed. (D and E) quantitative real‐time PCR and western blot verified the expression of PCK2 and FBP1 in HT29 cells with or without depleting AF9 or HCT116 cells with or without forced expression of AF9. (F) The key metabolites in the glycolytic pathway were quantified by mass in HT29 cells with or without depleting AF9 or HCT116 cells with or without forced expression of AF9. (G) The cells were cultured with the cell density reaching 50%, and extracellular acidification rate (ECAR) was measured by the XF glycolysis stress test. (H) Representative fluoro2‐D‐deoxyglucose F18 (18F‐FDG) PET/computed tomography images of CRC patients with low or high AF9 expression; difference analysis of standardised uptake value (SUV)max in the AF9‐high and AF9‐low groups (*p < .05, **p < .01, ***p < .001, ****p < .0001).