Figure 1.

CAMP is upregulated in circulating neutrophils and ischemic brain. (a–b) Quantification of CAMP in the plasma of male (a) and female (b) stroke patients within 3 days by ELISA (n = 17-23 per group, unpaired Student’s t-test). (c–d) Quantification of CAMP in the plasma of MCAO male mice (c) and female mice (d) at 1, 3, 7 and 14 days after MCAO (n = 6 per group, one-way ANOVA with Bonferroni multiple comparisons test). (e) Representative flow cytometry plots for CAMP⁺ Ly6G⁺ neutrophils in the peripheral blood of MCAO mice at 1 and 3 days after MCAO. (f–i) Quantification of CAMP⁺ Ly6G⁺ neutrophils cell numbers and percentage, total neutrophils prcentage and CAMP MFI in the peripheral blood at 1 and 3 days after MCAO (n = 3 per group, one-way ANOVA with Bonferroni multiple comparisons test). (j) Representative images showed expression of CAMP in neutrophils inflitrated in the infarct core at indicated timepoints after MCAO. The representative pictures of three individual mouse samples per group were shown. Scale bar, 20μm and (k–l) Quantification of CAMP⁺ Ly6G⁺ neutrophils counts per mm² and percentage in the infarct core at indicated timepoints after MCAO (n = 6 per group, one-way ANOVA with Bonferroni multiple comparisons test). All data are presented as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, no significance.